NoteNucleotide Sequences of Genes Encoding Allosamidin - sensitive Chitinases Produced by Allosamidin - producing Streptomyces JSma
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چکیده
Allosamidin is a strong inhibitor of family 18 chitinases. We preyiously reported the presence of allosamidin-sensitiye and -insensitive chitillases (chitinase S a"d IS) in the culture filtrate of the altosamidinproducing strain, Streptomyces sp. AJ9463. In this study, we cloned and sequenced the genes encoding the two chitinases, which clarified that chitinase S and IS belong to the family 18 and 19 chitinase, respectiyely.
منابع مشابه
Nucleotide sequences of genes encoding allosamidin-sensitive and -insensitive chitinases produced by allosamidin-producing Streptomyces.
Allosamidin is a strong inhibitor of family 18 chitinases. We previously reported the presence of allosamidin-sensitive and -insensitive chitinases (chitinase S and IS) in the culture filtrate of the allosamidin-producing strain, Streptomyces sp. AJ9463. In this study, we cloned and sequenced the genes encoding the two chitinases, which clarified that chitinase S and IS belong to the family 18 ...
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Allosamidin is a known inhibitor of class 18 chitinases. We show that allosamidin is a competitive inhibitor of the fungal chitinase CiX1 from Coccidioides immitis, with a K(i) of 60 nM. We report the X-ray structure of the complex and show that upon inhibitor binding the side-chain of Asp169 rotates to form an ion pair with the oxazolinium cation. The mechanism of action is thought to involve ...
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تاریخ انتشار 2018